Molecular Robotics and Molecular Computing by nanobiotechnology

We are challenging to construct autonomous nano/micrometer-sized “molecular robots” and “molecular computers”. Molecular robots and molecular computers are made of biopolymers and biomaterials such as DNA, protein, lipids, etc. The Molecular robots and molecular computers have autonomously working sensors, processors, and actuators. They can work in microscopic spaces like inside of cells and can be applied to medical systems, tiny machines, etc. See the following reviews and our published papers for detail:

  • Chikako Kurokawa, Kei Fujiwara, Masamune Morita, Ibuki Kawamata, Yui Kawagishi, Atsushi Sakai, Yoshihiro Murayama, Shin-ichiro M. Nomura, Satoshi Murata, *Masahiro Takinoue, *Miho Yanagisawa, “DNA cytoskeleton for stabilizing artificial cells”, Proc. Natl. Acad. Sci. USA (PNAS), vol.114, no.28, pp.7228-7233, (2017). doi: 10.1073/pnas.1702208114. link
  • Masayuki Hayakawa, Hiroaki Onoe, Ken H. Nagai, *Masahiro Takinoue, “Complex-shaped three-dimensional multi-compartmental microparticles generated by diffusional and Marangoni microflows in centrifugally discharged droplets”, Sci. Rep., vol. 6, 20793 (2016). DOI: 10.1038/srep20793 link
  • S. Murata, A. Konagaya, S. Kobayashi, H. Saito, M. Hagiya, “Molecular Robotics: A New Paradigm for Artifacts”, New Generation Computing (Ohmsha, Ltd. and Springer), 31, 27-45 (2013). link
  • Masahiro Takinoue, Akira Suyama, “Hairpin-DNA Memory Using Molecular Addressing”, Small, vol. 2, pp. 1244-1247 (2006). link

Artificial Cell and Nonlinear Non-equilibrium Science with Microfluidics

We are constructing “artificial cells” using biomolecules (DNA, RNA, protein, lipid, etc.) to understand essential aspect of life systems. Life systems exhibit dynamical behavior such as autonomous sensing, autonomous information processing, autonomous motion, etc. Artificially constructing cell-like systems is important not only for basic science (physics, chemistry, biology) but also for applied science and engineering. See the following review and our published papers for detail:

  • Haruka Sugiura, Manami Ito, Tomoya Okuaki, Yoshihito Mori, Hiroyuki Kitahata, *Masahiro Takinoue, “Pulse-density modulation control of chemical oscillation far from equilibrium in a droplet open-reactor system”, Nature Commun., vol.7, 10212 (2016). DOI: 10.1038/ncomms10212 link (SI)
    Press release: [English]
  • Masayuki Hayakawa, Satoshi Umeyama, Ken H. Nagai, Hiroaki Onoe, *Masahiro Takinoue, “Controlled construction of stable network structure composed of honeycomb-shaped microhydrogels”, Life, vol.8, no.4, article number 38 (2018). link
  • Masamune Morita, Hiroaki Onoe, Miho Yanagisawa, Hiroaki Ito, Masatoshi Ichikawa, Kei Fujiwara, Hirohide Saito, *Masahiro Takinoue, “Droplet-Shooting and Size-Filtration (DSSF) Method for Synthesis of Cell-Sized Liposomes with Controlled Lipid Compositions”, ChemBioChem, vol. 16, no. 14, pp. 2029-2035 (2015). DOI: 10.1002/cbic.201500354 link
  • Masahiro Takinoue, Shoji Takeuchi, “Droplet microfluidics for the study of artificial cells”, Anal. Bioanal. Chem., vol. 400, pp. 1705-1716 (2011). link